You have isolated mRNA corresponding to a protein kinase involved in human cancer. Your ultimate goa Show more You have isolated mRNA corresponding to a protein kinase involved in human cancer. Your ultimate goal is to study the properties of this kinase in an enzymatic assay to understand its function. You will use the tools of recombinant technology and molecular biology to produce a purified sample of this protein. Starting from the mRNA describe a detailed chronological list of all the steps you will follow to obtain a pure protein sample. For each step, describe the rationale/function of the process used. Show less
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